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_STwo different worlds but same struggles 😮💨
#phdlife #phdstudent #wetlab #drylab #bioinformatics
@_sukhraj._.singh_
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PR🫡🤷♀️
#lab #labmemes #research #researchlife #science #wetlab #experiments #careerchoices
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PYIs this for real 😮
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THAlways happens 😔 #officelife #sciencememes #mcmasteru #wetlab #genz
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THThe Top 5 Most Useful Lab Formulas for Microbiologists & Molecular Biologists:
Save this for your next experiment! 📚✨
1️⃣ C₁V₁ = C₂V₂ – Dilution/Concentration Formula
This formula helps you calculate how much of a concentrated stock solution you need to dilute to reach a desired final concentration and volume. It ensures accuracy in preparing buffers, antibiotics, primers, and any reagent that requires working concentrations from concentrated stocks.
2️⃣ CFU/mL = (Colonies × Dilution Factor) / Volume Plated – Bacterial Concentration Formula
Used to estimate the number of viable bacteria in a sample. After serial dilution and plating, this formula quantifies the density of cells in the original culture. It's crucial for understanding bacterial growth, standardizing inoculums, or evaluating antimicrobial effects.
3️⃣ Molarity (M) = Mass (g) / (Molar Mass × Volume in Liters) – Solution Preparation Formula
This is essential for preparing solutions at specific molar concentrations. Knowing molarity allows you to prepare reagents with precise chemical strength, ensuring reproducibility and consistency in experiments involving buffers, salts, and substrates.
4️⃣ Dilution Factor = Volume of Sample / (Volume of Sample + Volume of Diluent) – Serial Dilution Formula
This tells you how much your sample is diluted. It’s especially useful when performing serial dilutions for plating, enzyme reactions, or quantitative assays. Understanding dilution factors ensures accurate concentration gradients for downstream analysis.
5️⃣ % (w/v) = (Mass of Solute / Volume of Solution) × 100 – Percentage Weight/Volume Formula
This formula is widely used to prepare common lab solutions such as sugar, salt, and detergent solutions. It defines the concentration of a solute in a given volume and is standard for solid-in-liquid preparations where molarity may not be practical or needed.
⚠️ Bookmark this 📌 for future use 👩🔬
#PhDLife #LabFormulas #MolecularBiology #MicrobiologyLab #ScienceReels #WetLab
#microbiology #biotechnology #formula #LabLife #laboratory #thinkbiology #thinkvision
Do you find it useful?👩🔬👨🔬
@thinkbiology_think_vision
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THFollow🎖️🧫 Cell Counting Made Simple | Hemocytometer Basics
Ever wondered how researchers accurately count cells before seeding or experiments? Here’s a clear, lab-friendly breakdown 👇
(Always follow your lab SOP — steps may slightly vary)
🔬 Step 1: Prepare your cells
• Adherent cells → Detach properly, make a single-cell suspension, then centrifuge
• Suspension cells → Directly centrifuge
• Resuspend the pellet in 0.5–1 mL fresh culture medium
🧼 Step 2: Prep the hemocytometer
• Clean and dry the hemocytometer and coverslip properly
• Place the coverslip correctly to form the counting chamber
🧪 Step 3: Staining
• Mix 10 µL cell suspension + 10 µL Trypan Blue
• This gives a dilution factor of 2
🧬 Step 4: Load & focus
• Load 10 µL of the mixture into the chamber
• Focus first at low magnification, then zoom in
🔢 Step 5: Counting
• Count cells in the four corner squares
• Count only live cells (clear/unstained)
• Dead cells appear blue
📐 Formula to remember
Cells/mL = Average cell count × Dilution factor × 10⁴
• Dilution factor here = 2
📝 Why this matters
✔️ Accurate cell seeding
✔️ Assess viability of newly thawed cultures
✔️ Maintain healthy, reproducible experiments
📌 Mastering cell counting = mastering basic cell culture skills.
Credit: Mahmoud
#CellCulture #Hemocytometer #LabLife #ResearchBasics #BiotechLab #MolecularBiology #CellCounting #WetLab #LifeScience #PhDLife #ThatCoolProfessor
@thatcoolprofessor
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VIThrilled to visit Catalyst with Seeker Labs this afternoon, a cutting-edge #wetlab facility in Toronto, built to fuel innovation and accelerate #lifesciences research!
This world-class facility offers scientists and innovators an inspiring place to turn big ideas into real-world breakthroughs. 🌍
Exciting to tour this space where the next generation of Ontario-made discoveries will take root!
@vicfedeli
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ELBorn to pipette🥰Forced to analyze😭
#scientistlife #wetlab #dataanalysis #stem #researcher
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_Bbonus points if you spot the slug 🤮🤣
#hedoesntknowityet #doesntknow #labrador #wetlab #muddypaws #muddydog #blacklab #bigmarshall #fyp
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PACheck your calendars folks
#doctor #residentdoctor #medicine #residency #ophthalmology #wetlab #cataract #cataractsurgery #eye #eyeball #doctorwho #wearfigs
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SPMembers from the 52nd Medical Group Alcohol and Drug Abuse Prevention and Treatment (ADAPT) offer #WETLAB Demonstrations to different units on base to educate about alcohol awareness. Members attending learn about the single serving size amounts for different types of alcohol, and take breathalyzer tests to see how much of an effect the alcohol has. (All members participating are of legal drinking age).
#ADAPT provides services to those who are experiencing problems with substance abuse or misuse.
For more information about ADAPT or to schedule a WET LAB call 06565-61-8321.
@@usairforce | @usafe_afafrica
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