2.5K
SOIf only qPCRs were that quick to set up in real life!!😂
#qpcr #experiment #lab #scientist #science
@sophinvitro
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91.5K
PHqPCR analysis made simple in Excel 🧬
Step-by-step workflow:
✔️ Average replicates
✔️ ΔCq = GOI – Reference
✔️ ΔΔCq = Sample – Control
✔️ Fold change = 2^-ΔΔCq
💬 Comment qPCR if you want the Excel sheet template!
#qpcr #molecularbiology #excel #research #phd biotechnology gene expression lab womeninscience
@phdwithanjali
4.4K
NELet’s run a qPCR plate together!!
Although only going through the final stage of qPCR to be able to run your qPCR you will need to extract RNA from tissue/cells and concert to cDNA before running the qPCR reaction!
To run a qPCR you need to remember:
1. Have a housekeeping gene alongside experiment genes e.g Beta actin
2. Contamination is your enemy! Keep everything clean with ethanol and DNA/RNase zap spray
3. Changing tips when changing sample
4. Ensure thawing on ice for all of your reagents/samples/primers - if in doubt put it on ice #phd #wetlab #qpcr #molecularbiology #neuroscience
@neurosciencewithsof
99.0K
APstill trying to optimize one of my primer pairs but I think I’m getting closer🤞🏼
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#phdlife #phd #yale #yaleuniversity #gradstudent #graduatestudent #phdstudent #phdstudentlife #lablife #gmo #biology #academia #researchlife #plantscientist #stem #womeninstem #womeninscience #plantbiology #molecularbiology #biochem #plantbiologist #qpcr #PCR #experiment
@aplantbiologist
51.4K
DRUnlock the secrets of qPCR! 🧬 Dive into the world of melt curve analysis and master your real-time qPCR results. Are you ready to become a qPCR pro? 🔬 #ScienceSimplified #qPCRmastery
@drpriyanshu.insights
437.8K
C.We’ve all been there 🤣😭
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#biologymemes #biology #qpcr #research #researchassistant #labmemes #lablife #phdlife #phd #motivation #productivity #university #gradschool #science
@c.belliveau.science
120.3K
THI miscalculated so only filled half the plate but still nearly took nearly an hour 🥲
#womeninstem #stem #phd #science #scientist #universityofcambridge #biology #neuroscience #fyp #biochemistry #fypシ #pcr #qpcr #cambridge #phdstudent #phdlife
@thosephdgirls
27.5K
AB🙄 REPEATED EXPERIMENTS 🙄
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This is for a very niche audience who understand the pain behind qPCR 😭🥲
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1 year and a half in - and I’m repeating it all for what feels like the 5 billionth time because it keeps MESSING UP 😤
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If we didn’t laugh we’d cry ehhh? 😅✌🏼
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#stress #phdlife #phdstudent #gradschoollife #stressedstudent #phdjourney #phdproblems #life #universitylife #researcherlife #womeninstem #science #sciencefail
@abioblog
14.2K
CABeyond eyeball-ing your cells 👀🧫
Here are actual ways you can verify your cells are what they claim to be 🧐🤔
Start with what you can check in-house:
✨qPCR or western blot to check for expression of specific markers
✨ Immunofluorescence to check expression of specific markers and confirm homogeneous cell population
✨If your cells are mixed population, you can try to perform FACS (flow cytometry + cell sorting) to select for your desired cells. However, this only works if your protocol doesn’t require fixation (which kills cells) and specific markers are extracellular (intracellular requires permeabilisation which kills cells).
You can also send your cell lines to cell banks, where they can perform short tandem repeats (STR) profiling to identify your cell lines.
Run your experiments with confidence and it starts with knowing your cells truly.
Stay tuned for the next episode 📢
#phdlife #phd #gradschool #biology #stem #labtok #science #lablife #phdmemes #research #labhacks
@capinpinpin
211
SCqPCR is a research procedure that uses fluorescence to measure how much DNA (nucleic acid) is in a sample. Currently, it is the gold standard for COVID-19 testing.
#science #scienceeducation #sciencefacts #qpcr #laboratory #experiment #minipcr
@science.education.dept
15.0K
THPutting the q in qPCR: a practical look at analyzing quantitative PCR data (and RealTime PCR in general)
blog: https://bit.ly/qpcranalysis ; YouTube: https://youtu.be/4WPkAMB_dPU
I’ve talked a lot about the theoretical basis for these techniques - using PCR to make lots of copies on a sequence, using fluorescence to measure the copies as they’re made (i.e in Real Time), and seeing how fast the fluorescence rises - but today I want to show you the practical aspects of using that rise in fluorescence to determine how many copies you started with.
And, before you get jumbled up in alphabet soup, note that RT can stand for real-time or reverse transcription. Because RT—RT-PCR is often used to measure “gene expression” by converting mRNA to complementary DNA (cDNA) then measuring levels of those copies.
Another confusing thing is that real-time PCR isn’t always truly quantitative. If you want an absolute (as opposed to relative) concentration, you need to include a standard curve of known concentration of the product. Then you compare to that. In order to compare, you need to be in the linear range of detection. So you may need to dilute your sample before running (if you aren’t sure what how much you need to dilute it, test out a few different dilution factors). If you do dilute, be sure to take this into account when calculating the concentration you get from your standard curve equation. For example, if you did a 1:4 dilution, multiply the concentration you calculate by 4 to get your initial, “stock” concentration.
Yet another confusing thing is that the terms Cq and Ct are used interchangeably to refer to the cycle number at which the fluorescence increases above the “threshold” - the signal from background. With either, the lower the value, the higher the concentration.
Hopefully the video helps explain these things better and here is a link to the example if you wanted to follow along: https://bit.ly/qpcr_analysis_example PART 2 👇
@thebumblingbiochemist
19.7K
LANão se deixe levar pela pressa. Escolha a perfeição! 🧬
Estamos mais do que prontos para iniciar nesta semana nossas atividades em nosso setor de biologia molecular, e muito animados em oferecer exames de qualidade, confiança e com diagnósticos precisos!
Vem com a gente em mais uma novidade Animalex!
#laboratorioveterinario #analisesclinicas #diagnosticoveterinario #biologiamolecular #qpcr #medvet #df
@lab_animalex
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