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BIwhat's your favorite application of CRISPR, and does it echo through the molecular gloving community? credit for footage: nature video CRISPR: Gene editing and beyond, Liquid FlowArt, zac.gloves .
Park SJ, Lee GE, Cho SM, Choi EH. Recent applications, future perspectives, and limitations of the CRISPR-Cas system. Mol Ther Nucleic Acids. 2025 Jul 17;36(3):102634.
"Although originally characterized as an adaptive immune system in bacteria and archaea, CRISPR-Cas9 has transcended its native role to revolutionize fields ranging from functional genomics to translational medicine.
Pivotal observations of mysterious repeat sequences in the Escherichia coli genome in 1987 laid the foundation for this paradigm shift, yet it was not until the early 2000s that these loci, now known as CRISPR arrays, were recognized as evidences of past viral encounters and the crucible of a heritable defense system.
By utilizing a single effector protein and a customizable guide RNA (gRNA), CRISPR-Cas9 surpasses earlier engineered nucleases such as zinc-finger nucleases and transcription activator-like effector nucleases in simplicity and flexibility.
This efficient architecture has catalyzed rapid adoption across diverse organisms and cell types, facilitating both loss-of-function screens and precise gene corrections. Central to its diverse function is the cellular response to Cas9-induced double-strand breaks (DSBs), which are resolved by competing repair pathways, primarily error-prone non-homologous end joining (NHEJ) and high-fidelity homology-directed repair (HDR)."
#dna #genetics #crispr #genome #geneediting
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